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Biosurfactants produced by Microbacterium sp., isolated from aquatic macrophytes in hydrocarbon-contaminated area in the Rio Negro, Manaus, Amazonas João Marcelo Silva Lima, Jose Odair Pereira, Ieda Hortêncio Batista, Raimundo Carlos Pereira Junior, Hileia dos Santos Barroso, Pedro de Queiroz Costa Neto, Ani Beatriz Jackisch Matsuura, Suzelei de Castro França, João Lúcio Azevedo Resumo Endophytic bacteria isolated from Eichhornia crassipes (Mart) Solms., collected in oil contaminated wastewater of effluent generated by Petrobras refinery in Manaus were investigated to determine their potential for producing biosurfactants. Assay with 2.6-dichlorophenol indophenol (DCPIP) indicator to verify hydrocarbon biodegradation activity; oil emulsification test; drop-collapse method; surface tension and growth curve of biosurfactant production. The M87 Microbacterium sp. strain chosen for this work was identified by the sequencing of the rDNA region and the chemical characterization was p...